AG Superresolution Microscopy

2024

Shrestha D, Bahasoan Y, Eggeling C. Cellular Output and Physicochemical
Properties of the Membrane-Derived Vesicles Depend on Chemical Stimulants. ACS
Appl Mater Interfaces. 2024 Sep 18;16(37):48982-48992. doi:
10.1021/acsami.4c07234. Epub 2024 Sep 9. PMID: 39250321; PMCID: PMC11420866.

Prakash K, Baddeley D, Eggeling C, Fiolka R, Heintzmann R, Manley S,
Radenovic A, Smith C, Shroff H, Schermelleh L. Resolution in super-resolution
microscopy - definition, trade-offs and perspectives. Nat Rev Mol Cell Biol.
2024 Sep;25(9):677-682. doi: 10.1038/s41580-024-00755-7. Epub 2024 Jul 1. PMID:
38951703.

Dasgupta A, Koerfer A, Kokot B, Urbančič I, Eggeling C, Carravilla P. Effects
and avoidance of photoconversion-induced artifacts in confocal and STED
microscopy. Nat Methods. 2024 Jul;21(7):1171-1174. doi:
10.1038/s41592-024-02297-4. Epub 2024 Jun 4. PMID: 38834747.

Alonso-Roman R, Mosig AS, Figge MT, Papenfort K, Eggeling C, Schacher FH,
Hube B, Gresnigt MS. Organ-on-chip models for infectious disease research. Nat
Microbiol. 2024 Apr;9(4):891-904. doi: 10.1038/s41564-024-01645-6. Epub 2024 Mar
25. PMID: 38528150.

Seltmann A, Carravilla P, Reglinski K, Eggeling C, Waithe D. Neural network
informed photon filtering reduces fluorescence correlation spectroscopy
artifacts. Biophys J. 2024 Mar 19;123(6):745-755. doi:
10.1016/j.bpj.2024.02.012. Epub 2024 Feb 20. PMID: 38384131; PMCID: PMC10995453.

Valentine M, Rudolph P, Dietschmann A, Tsavou A, Mogavero S, Lee S, Priest
EL, Zhurgenbayeva G, Jablonowski N, Timme S, Eggeling C, Allert S, Dolk E,
Naglik JR, Figge MT, Gresnigt MS, Hube B. Nanobody-mediated neutralization of
candidalysin prevents epithelial damage and inflammatory responses that drive
vulvovaginal candidiasis pathogenesis. mBio. 2024 Mar 13;15(3):e0340923. doi:
10.1128/mbio.03409-23. Epub 2024 Feb 13. PMID: 38349176; PMCID: PMC10936171.

Kowalczuk K, Dasgupta A, Páez Larios F, Ulrich HF, Wegner V, Brendel JC,
Eggeling C, Mosig AS, Schacher FH. Self-Degrading Multifunctional PEG-Based
Hydrogels-Tailormade Substrates for Cell Culture. Macromol Biosci. 2024
May;24(5):e2300383. doi: 10.1002/mabi.202300383. Epub 2024 Jan 2. PMID:
38102978.

2023

Vogler BTL, Reina F, Eggeling C. Blob-B-Gone: a lightweight framework for
removing blob artifacts from 2D/3D MINFLUX single-particle tracking data. Front
Bioinform. 2023 Nov 22;3:1268899. doi: 10.3389/fbinf.2023.1268899. PMID:
38076029; PMCID: PMC10704905.

Stark SL, Gross H, Reglinski K, Messerschmidt B, Eggeling C. Field curvature
reduction in miniaturized high numerical aperture and large field-of-view
objective lenses with sub 1 µm lateral resolution. Biomed Opt Express. 2023 Nov
7;14(12):6190-6205. doi: 10.1364/BOE.499785. PMID: 38420300; PMCID: PMC10898576.

Koerfer A, Reina F, Eggeling C. Influence of different surface cleaning
methods on STED-FCS and scanning STED-FCS calibration measurements. J Microsc.
2023 Dec 6. doi: 10.1111/jmi.13250. Epub ahead of print. PMID: 38054327.

11: Svensson CM, Reglinski K, Schliebs W, Erdmann R, Eggeling C, Figge MT.
Quantitative analysis of peroxisome tracks using a Hidden Markov Model. Sci Rep.
2023 Nov 11;13(1):19694. doi: 10.1038/s41598-023-46812-7. PMID: 37951993; PMCID:
PMC10640649.

Reinhard J, Kaleta S, Abel JJ, Wiesner F, Wünsche M, Seemann E, Westermann
M, Weber T, Nathanael J, Iliou A, Fiedorowicz H, Hillmann F, Eggeling C, Paulus
GG, Fuchs S. Laboratory-Based Correlative Soft X-ray and Fluorescence Microscopy
in an Integrated Setup. Microsc Microanal. 2023 Dec 21;29(6):2014-2025. doi:
10.1093/micmic/ozad123. PMID: 37944034.

Joseph J, Spantzel L, Ali M, Moonnukandathil Joseph D, Unger S, Reglinski K,
Krafft C, Müller AD, Eggeling C, Heintzmann R, Börsch M, Press AT, Täuber D.
Nanoscale chemical characterization of secondary protein structure of F-Actin
using mid-infrared photoinduced force microscopy (PiF-IR). Spectrochim Acta A
Mol Biomol Spectrosc. 2024 Feb 5;306:123612. doi: 10.1016/j.saa.2023.123612.
Epub 2023 Nov 3. PMID: 37931494.

Hufsky F, Abecasis AB, Babaian A, Beck S, Brierley L, Dellicour S, Eggeling
C, Elena SF, Gieraths U, Ha AD, Harvey W, Jones TC, Lamkiewicz K, Lovate GL,
Lücking D, Machyna M, Nishimura L, Nocke MK, Renard BY, Sakaguchi S, Sakellaridi
L, Spangenberg J, Tarradas-Alemany M, Triebel S, Vakulenko Y, Wijesekara RY,
González-Candelas F, Krautwurst S, Pérez-Cataluña A, Randazzo W, Sánchez G, Marz
M. The International Virus Bioinformatics Meeting 2023. Viruses. 2023 Sep
30;15(10):2031. doi: 10.3390/v15102031. PMID: 37896809; PMCID: PMC10612056.

Ebrahimi V, Stephan T, Kim J, Carravilla P, Eggeling C, Jakobs S, Han KY.
Author Correction: Deep learning enables fast, gentle STED microscopy. Commun
Biol. 2023 Aug 10;6(1):831. doi: 10.1038/s42003-023-05222-1. Erratum for: Commun
Biol. 2023 Jun 27;6(1):674. doi: 10.1038/s42003-023-05054-z. PMID: 37563357;
PMCID: PMC10415319.

Ebrahimi V, Stephan T, Kim J, Carravilla P, Eggeling C, Jakobs S, Han KY.
Deep learning enables fast, gentle STED microscopy. Commun Biol. 2023 Jun
27;6(1):674. doi: 10.1038/s42003-023-05054-z. Erratum in: Commun Biol. 2023 Aug
10;6(1):831. doi: 10.1038/s42003-023-05222-1. PMID: 37369761; PMCID:
PMC10300082.

Urbančič I, Eggeling C, Sezgin E. Do lipids tune B cell signaling? Nat Chem
Biol. 2023 Jun;19(6):669-670. doi: 10.1038/s41589-023-01303-8. PMID: 36997648.

Frawley AT, Leslie KG, Wycisk V, Galiani S, Shrestha D, Eggeling C, Anderson
HL. A Photoswitchable Solvatochromic Dye for Probing Membrane Ordering by
RESOLFT Super-resolution Microscopy. Chemphyschem. 2023 Jun
15;24(12):e202300125. doi: 10.1002/cphc.202300125. Epub 2023 Apr 19. PMID:
36946252.

Ebrahimi V, Stephan T, Kim J, Carravilla P, Eggeling C, Jakobs S, Han KY.
Deep learning enables fast, gentle STED microscopy. bioRxiv [Preprint]. 2023 Jan
27:2023.01.26.525571. doi: 10.1101/2023.01.26.525571. Update in: Commun Biol.
2023 Jun 27;6(1):674. doi: 10.1038/s42003-023-05054-z. PMID: 36747618; PMCID:
PMC9900922.

Galiani S, Eggeling C, Reglinski K. Super-resolution microscopy and studies
of peroxisomes. Biol Chem. 2023 Jan 26;404(2-3):87-106. doi:
10.1515/hsz-2022-0314. PMID: 36698322.

Yang T, Valavalkar A, Romero-Arenas A, Dasgupta A, Then P, Chettri A,
Eggeling C, Ros A, Pischel U, Dietzek-Ivanšić B. Excited-State Dynamics in
Borylated Arylisoquinoline Complexes in Solution and in cellulo. Chemistry. 2023
Mar 16;29(16):e202203468. doi: 10.1002/chem.202203468. Epub 2023 Feb 14. PMID:
36477948.

Kühne M, Hofmann S, Lindemann H, Cseresnyés Z, Dzierza A, Schröder D,
Godmann M, Koschella A, Eggeling C, Fischer D, Figge MT, Heinze T, Heinzel T.
Analysis of HDACi-Coupled Nanoparticles: Opportunities and Challenges. Methods
Mol Biol. 2023;2589:129-144. doi: 10.1007/978-1-0716-2788-4_9. PMID: 36255622.

2022

Wallace Z, Kopycinski J, Yang H, McCully ML, Eggeling C, Chojnacki J,
Dorrell L. Immune mobilising T cell receptors redirect polyclonal
CD8⁺ T cells in chronic HIV infection to form immunological synapses.
Sci Rep. 2022 Nov 1;12(1):18366. doi: 10.1038/s41598-022-23228-3. PMID:
36319836; PMCID: PMC9626491.

Insausti S, Garcia-Porras M, Torralba J, Morillo I, Ramos-Caballero A, de la
Arada I, Apellaniz B, Caaveiro JMM, Carravilla P, Eggeling C, Rujas E, Nieva JL.
Functional Delineation of a Protein-Membrane Interaction Hotspot Site on the
HIV-1 Neutralizing Antibody 10E8. Int J Mol Sci. 2022 Sep 15;23(18):10767. doi:
10.3390/ijms231810767. PMID: 36142694; PMCID: PMC9504841.

Carravilla P, Dasgupta A, Zhurgenbayeva G, Danylchuk DI, Klymchenko AS,
Sezgin E, Eggeling C. Erratum: Long-term STED imaging of membrane packing and
dynamics by exchangeable polarity-sensitive dyes. Biophys Rep (N Y). 2022 Jun
8;2(2):None. doi: 10.1016/j.bpr.2022.100057. Erratum for: Biophys Rep (N Y).
2021 Dec 08;1(2):None. doi: 10.1016/j.bpr.2021.100023. PMID: 35712052; PMCID:
PMC9177438.

Reina F, Wigg JMA, Dmitrieva M, Vogler B, Lefebvre J, Rittscher J, Eggeling
C. TRAIT2D: a Software for Quantitative Analysis of Single Particle Diffusion
Data. F1000Res. 2021 Aug 20;10:838. doi: 10.12688/f1000research.54788.2. PMID:
35186271; PMCID: PMC8829092.

Portwich FL, Carstensen Y, Dasgupta A, Kupfer S, Wyrwa R, Görls H, Eggeling
C, Dietzek B, Gräfe S, Wächtler M, Kretschmer R. A Highly Fluorescent Dinuclear
Aluminium Complex with Near-Unity Quantum Yield. Angew Chem Int Ed Engl. 2022
Apr 19;61(17):e202117499. doi: 10.1002/anie.202117499. Epub 2022 Feb 28. PMID:
35107199; PMCID: PMC9313782.

2021

Kretzer C, Shkodra B, Klemm P, Jordan PM, Schröder D, Cinar G, Vollrath A,
Schubert S, Nischang I, Hoeppener S, Stumpf S, Banoglu E, Gladigau F, Bilancia
R, Rossi A, Eggeling C, Neugebauer U, Schubert US, Werz O. Ethoxy acetalated
dextran-based nanocarriers accomplish efficient inhibition of leukotriene
formation by a novel FLAP antagonist in human leukocytes and blood. Cell Mol
Life Sci. 2021 Dec 31;79(1):40. doi: 10.1007/s00018-021-04039-7. PMID: 34971430;
PMCID: PMC8966466.

Carravilla P, Dasgupta A, Zhurgenbayeva G, Danylchuk DI, Klymchenko AS,
Sezgin E, Eggeling C. Long-term STED imaging of membrane packing and dynamics by
exchangeable polarity-sensitive dyes. Biophys Rep (N Y). 2021 Dec 8;1(2):None.
doi: 10.1016/j.bpr.2021.100023. Erratum in: Biophys Rep (N Y). 2022 Jun
08;2(2):None. doi: 10.1016/j.bpr.2022.100057. PMID: 34939048; PMCID: PMC8651516.

Kokot B, Kokot H, Umek P, van Midden KP, Pajk S, Garvas M, Eggeling C,
Koklič T, Urbančič I, Štrancar J. How to control fluorescent labeling of metal
oxide nanoparticles for artefact-free live cell microscopy. Nanotoxicology. 2021
Oct;15(8):1102-1123. doi: 10.1080/17435390.2021.1973607. Epub 2021 Oct 6. PMID:
34612152.

Rujas E, Leaman DP, Insausti S, Carravilla P, García-Porras M, Largo E,
Morillo I, Sánchez-Eugenia R, Zhang L, Cui H, Iloro I, Elortza F, Julien JP,
Eggeling C, Zwick MB, Caaveiro JMM, Nieva JL. Focal accumulation of aromaticity
at the CDRH3 loop mitigates 4E10 polyreactivity without altering its HIV
neutralization profile. iScience. 2021 Aug 17;24(9):102987. doi:
10.1016/j.isci.2021.102987. PMID: 34505005; PMCID: PMC8413895.

Urbančič I, Schiffelers L, Jenkins E, Gong W, Santos AM, Schneider F,
O'Brien-Ball C, Vuong MT, Ashman N, Sezgin E, Eggeling C. Aggregation and
mobility of membrane proteins interplay with local lipid order in the plasma
membrane of T cells. FEBS Lett. 2021 Aug;595(16):2127-2146. doi:
10.1002/1873-3468.14153. Epub 2021 Jul 7. PMID: 34160065.

Chojnacki J, Eggeling C. Super-Resolution STED Microscopy-Based Mobility
Studies of the Viral Env Protein at HIV-1 Assembly Sites of Fully Infected
T-Cells. Viruses. 2021 Apr 2;13(4):608. doi: 10.3390/v13040608. PMID: 33918253;
PMCID: PMC8067239.

2020

Frawley AT, Wycisk V, Xiong Y, Galiani S, Sezgin E, Urbančič I, Vargas
Jentzsch A, Leslie KG, Eggeling C, Anderson HL. Super-resolution RESOLFT
microscopy of lipid bilayers using a fluorophore-switch dyad. Chem Sci. 2020 Aug
10;11(33):8955-8960. doi: 10.1039/d0sc02447c. PMID: 34123149; PMCID: PMC8163400.

Schneider F, Sych T, Eggeling C, Sezgin E. Influence of nanobody binding on
fluorescence emission, mobility, and organization of GFP-tagged proteins.
iScience. 2020 Dec 4;24(1):101891. doi: 10.1016/j.isci.2020.101891. PMID:
33364580; PMCID: PMC7753935.

Lühr JJ, Alex N, Amon L, Kräter M, Kubánková M, Sezgin E, Lehmann CHK, Heger
L, Heidkamp GF, Smith AS, Zaburdaev V, Böckmann RA, Levental I, Dustin ML,
Eggeling C, Guck J, Dudziak D. Maturation of Monocyte-Derived DCs Leads to
Increased Cellular Stiffness, Higher Membrane Fluidity, and Changed Lipid
Composition. Front Immunol. 2020 Nov 27;11:590121. doi:
10.3389/fimmu.2020.590121. PMID: 33329576; PMCID: PMC7728921.

Reglinski K, Steinfort-Effelsberg L, Sezgin E, Klose C, Platta HW, Girzalsky
W, Eggeling C, Erdmann R. Fluidity and Lipid Composition of Membranes of
Peroxisomes, Mitochondria and the ER From Oleic Acid-Induced <i>Saccharomyces
cerevisiae</i>. Front Cell Dev Biol. 2020 Oct 29;8:574363. doi:
10.3389/fcell.2020.574363. PMID: 33195209; PMCID: PMC7658010.

Gutowska-Owsiak D, Podobas EI, Eggeling C, Ogg GS, Bernardino de la Serna J.
Addressing Differentiation in Live Human Keratinocytes by Assessment of Membrane
Packing Order. Front Cell Dev Biol. 2020 Oct 21;8:573230. doi:
10.3389/fcell.2020.573230. PMID: 33195206; PMCID: PMC7609878.

Schneider F, Hernandez-Varas P, Christoffer Lagerholm B, Shrestha D, Sezgin
E, Julia Roberti M, Ossato G, Hecht F, Eggeling C, Urbančič I. High photon count
rates improve the quality of super-resolution fluorescence fluctuation
spectroscopy. J Phys D Appl Phys. 2020 Apr 15;53(16):164003. doi:
10.1088/1361-6463/ab6cca. Epub 2020 Feb 13. PMID: 33191951; PMCID: PMC7655148.

Büttner M, Lagerholm CB, Waithe D, Galiani S, Schliebs W, Erdmann R,
Eggeling C, Reglinski K. Challenges of Using Expansion Microscopy for Super-
resolved Imaging of Cellular Organelles. Chembiochem. 2021 Feb 15;22(4):686-693.
doi: 10.1002/cbic.202000571. Epub 2020 Nov 11. PMID: 33049107; PMCID:
PMC7894168.

Kühne M, Lindemann H, Grune C, Schröder D, Cseresnyés Z, Godmann M,
Koschella A, Figge MT, Eggeling C, Fischer D, Heinze T, Heinzel T. Biocompatible
sulfated valproic acid-coupled polysaccharide-based nanocarriers with HDAC
inhibitory activity. J Control Release. 2021 Jan 10;329:717-730. doi:
10.1016/j.jconrel.2020.10.006. Epub 2020 Oct 5. PMID: 33031880.

Waithe D, Brown JM, Reglinski K, Diez-Sevilla I, Roberts D, Eggeling C.
Object detection networks and augmented reality for cellular detection in
fluorescence microscopy. J Cell Biol. 2020 Oct 5;219(10):e201903166. doi:
10.1083/jcb.201903166. PMID: 32854116; PMCID: PMC7659718.

Rujas E, Insausti S, Leaman DP, Carravilla P, González-Resines S, Monceaux
V, Sánchez-Eugenia R, García-Porras M, Iloro I, Zhang L, Elortza F, Julien JP,
Saéz-Cirión A, Zwick MB, Eggeling C, Ojida A, Domene C, Caaveiro JMM, Nieva JL.
Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane
Environments. Cell Rep. 2020 Aug 18;32(7):108037. doi:
10.1016/j.celrep.2020.108037. PMID: 32814041; PMCID: PMC7861656.

Schmidt F, Thywißen A, Goldmann M, Cunha C, Cseresnyés Z, Schmidt H, Rafiq
M, Galiani S, Gräler MH, Chamilos G, Lacerda JF, Campos A Jr, Eggeling C, Figge
MT, Heinekamp T, Filler SG, Carvalho A, Brakhage AA. Flotillin-Dependent
Membrane Microdomains Are Required for Functional Phagolysosomes against Fungal
Infections. Cell Rep. 2020 Aug 18;32(7):108017. doi:
10.1016/j.celrep.2020.108017. PMID: 32814035; PMCID: PMC10054021.

Schneider KRA, Chettri A, Cole HD, Reglinski K, Brückmann J, Roque JA 3rd,
Stumper A, Nauroozi D, Schmid S, Lagerholm CB, Rau S, Bäuerle P, Eggeling C,
Cameron CG, McFarland SA, Dietzek B. Intracellular Photophysics of an Osmium
Complex bearing an Oligothiophene Extended Ligand. Chemistry. 2020 Nov
20;26(65):14844-14851. doi: 10.1002/chem.202002667. Epub 2020 Oct 14. PMID:
32761643; PMCID: PMC7704931.

Barbotin A, Urbančič I, Galiani S, Eggeling C, Booth M. Background Reduction
in STED-FCS Using a Bivortex Phase Mask. ACS Photonics. 2020 Jul
15;7(7):1742-1753. doi: 10.1021/acsphotonics.0c00388. Epub 2020 Jun 4. PMID:
32685609; PMCID: PMC7366504.

Sieben C, Sezgin E, Eggeling C, Manley S. Influenza A viruses use
multivalent sialic acid clusters for cell binding and receptor activation. PLoS
Pathog. 2020 Jul 8;16(7):e1008656. doi: 10.1371/journal.ppat.1008656. PMID:
32639985; PMCID: PMC7371231.

Torralba J, de la Arada I, Carravilla P, Insausti S, Rujas E, Largo E,
Eggeling C, Arrondo JLR, Apellániz B, Nieva JL. Cholesterol Constrains the
Antigenic Configuration of the Membrane-Proximal Neutralizing HIV-1 Epitope. ACS
Infect Dis. 2020 Aug 14;6(8):2155-2168. doi: 10.1021/acsinfecdis.0c00243. Epub
2020 Jul 8. PMID: 32584020.

Tröger J, Hoischen C, Perner B, Monajembashi S, Barbotin A, Löschberger A,
Eggeling C, Kessels MM, Qualmann B, Hemmerich P. Comparison of Multiscale
Imaging Methods for Brain Research. Cells. 2020 Jun 1;9(6):1377. doi:
10.3390/cells9061377. PMID: 32492970; PMCID: PMC7349602.

Barbotin A, Urbančič I, Galiani S, Eggeling C, Booth M, Sezgin E. z-STED
Imaging and Spectroscopy to Investigate Nanoscale Membrane Structure and
Dynamics. Biophys J. 2020 May 19;118(10):2448-2457. doi:
10.1016/j.bpj.2020.04.006. Epub 2020 Apr 16. PMID: 32359408; PMCID: PMC7231928.

Carravilla P, Nieva JL, Eggeling C. Fluorescence Microscopy of the HIV-1
Envelope. Viruses. 2020 Mar 21;12(3):348. doi: 10.3390/v12030348. PMID:
32245254; PMCID: PMC7150788.

Sezgin E, Carugo D, Levental I, Stride E, Eggeling C. Creating Supported
Plasma Membrane Bilayers Using Acoustic Pressure. Membranes (Basel). 2020 Feb
18;10(2):30. doi: 10.3390/membranes10020030. PMID: 32085393; PMCID: PMC7074417.

Stanly TA, Fritzsche M, Banerji S, Shrestha D, Schneider F, Eggeling C,
Jackson DG. The cortical actin network regulates avidity-dependent binding of
hyaluronan by the lymphatic vessel endothelial receptor LYVE-1. J Biol Chem.
2020 Apr 10;295(15):5036-5050. doi: 10.1074/jbc.RA119.011992. Epub 2020 Feb 7.
PMID: 32034091; PMCID: PMC7152780.

2019

Azbazdar Y, Ozalp O, Sezgin E, Veerapathiran S, Duncan AL, Sansom MSP,
Eggeling C, Wohland T, Karaca E, Ozhan G. More Favorable Palmitic Acid Over
Palmitoleic Acid Modification of Wnt3 Ensures Its Localization and Activity in
Plasma Membrane Domains. Front Cell Dev Biol. 2019 Nov 15;7:281. doi:
10.3389/fcell.2019.00281. PMID: 31803740; PMCID: PMC6873803.

Bedard M, Shrestha D, Priestman DA, Wang Y, Schneider F, Matute JD, Iyer SS,
Gileadi U, Prota G, Kandasamy M, Veerapen N, Besra G, Fritzsche M, Zeissig S,
Shevchenko A, Christianson JC, Platt FM, Eggeling C, Blumberg RS, Salio M,
Cerundolo V. Sterile activation of invariant natural killer T cells by ER-
stressed antigen-presenting cells. Proc Natl Acad Sci U S A. 2019 Nov
19;116(47):23671-23681. doi: 10.1073/pnas.1910097116. Epub 2019 Nov 5. PMID:
31690657; PMCID: PMC6876220.

Steinkühler J, Sezgin E, Urbančič I, Eggeling C, Dimova R. Mechanical
properties of plasma membrane vesicles correlate with lipid order, viscosity and
cell density. Commun Biol. 2019 Sep 13;2:337. doi: 10.1038/s42003-019-0583-3.
PMID: 31531398; PMCID: PMC6744421.

Barbotin A, Galiani S, Urbančič I, Eggeling C, Booth MJ. Adaptive optics
allows STED-FCS measurements in the cytoplasm of living cells. Opt Express. 2019
Aug 5;27(16):23378-23395. doi: 10.1364/OE.27.023378. PMID: 31510616; PMCID:
PMC6825603.

Pinkwart K, Schneider F, Lukoseviciute M, Sauka-Spengler T, Lyman E,
Eggeling C, Sezgin E. Nanoscale dynamics of cholesterol in the cell membrane. J
Biol Chem. 2019 Aug 23;294(34):12599-12609. doi: 10.1074/jbc.RA119.009683. Epub
2019 Jul 3. PMID: 31270209; PMCID: PMC6709632.

Colin-York H, Javanmardi Y, Skamrahl M, Kumari S, Chang VT, Khuon S, Taylor
A, Chew TL, Betzig E, Moeendarbary E, Cerundolo V, Eggeling C, Fritzsche M.
Cytoskeletal Control of Antigen-Dependent T Cell Activation. Cell Rep. 2019 Mar
19;26(12):3369-3379.e5. doi: 10.1016/j.celrep.2019.02.074. PMID: 30893608;
PMCID: PMC6426652.

Colin-York H, Li D, Korobchevskaya K, Chang VT, Betzig E, Eggeling C,
Fritzsche M. Cytoskeletal actin patterns shape mast cell activation. Commun
Biol. 2019 Mar 7;2:93. doi: 10.1038/s42003-019-0322-9. PMID: 30854485; PMCID:
PMC6405992.

Sezgin E, Schneider F, Galiani S, Urbančič I, Waithe D, Lagerholm BC,
Eggeling C. Measuring nanoscale diffusion dynamics in cellular membranes with
super-resolution STED-FCS. Nat Protoc. 2019 Apr;14(4):1054-1083. doi:
10.1038/s41596-019-0127-9. Epub 2019 Mar 6. PMID: 30842616.

Ando T, Bhamidimarri SP, Brending N, Colin-York H, Collinson L, De Jonge N,
de Pablo PJ, Debroye E, Eggeling C, Franck C, Fritzsche M, Gerritsen H, Giepmans
BNG, Grunewald K, Hofkens J, Hoogenboom JP, Janssen KPF, Kaufman R, Klumpermann
J, Kurniawan N, Kusch J, Liv N, Parekh V, Peckys DB, Rehfeldt F, Reutens DC,
Roeffaers MBJ, Salditt T, Schaap IAT, Schwarz US, Verkade P, Vogel MW, Wagner R,
Winterhalter M, Yuan H, Zifarelli G. The 2018 correlative microscopy techniques
roadmap. J Phys D Appl Phys. 2018 Nov 7;51(44):443001. doi:
10.1088/1361-6463/aad055. Epub 2018 Aug 31. PMID: 30799880; PMCID: PMC6372154.

Carravilla P, Chojnacki J, Rujas E, Insausti S, Largo E, Waithe D, Apellaniz
B, Sicard T, Julien JP, Eggeling C, Nieva JL. Molecular recognition of the
native HIV-1 MPER revealed by STED microscopy of single virions. Nat Commun.
2019 Jan 8;10(1):78. doi: 10.1038/s41467-018-07962-9. PMID: 30622256; PMCID:
PMC6325134.

Schermelleh L, Ferrand A, Huser T, Eggeling C, Sauer M, Biehlmaier O,
Drummen GPC. Super-resolution microscopy demystified. Nat Cell Biol. 2019
Jan;21(1):72-84. doi: 10.1038/s41556-018-0251-8. Epub 2019 Jan 2. PMID:
30602772.

2018

Felce JH, Sezgin E, Wane M, Brouwer H, Dustin ML, Eggeling C, Davis SJ. CD45
exclusion- and cross-linking-based receptor signaling together broaden FcεRI
reactivity. Sci Signal. 2018 Dec 18;11(561):eaat0756. doi:
10.1126/scisignal.aat0756. PMID: 30563863; PMCID: PMC7612966.

Jenkins E, Santos AM, O'Brien-Ball C, Felce JH, Wilcock MJ, Hatherley D,
Dustin ML, Davis SJ, Eggeling C, Sezgin E. Reconstitution of immune cell
interactions in free-standing membranes. J Cell Sci. 2018 Oct
2;132(4):jcs219709. doi: 10.1242/jcs.219709. PMID: 30209137; PMCID: PMC6398472.

Urbančič I, Brun J, Shrestha D, Waithe D, Eggeling C, Chojnacki J. Lipid
Composition but Not Curvature Is the Determinant Factor for the Low Molecular
Mobility Observed on the Membrane of Virus-Like Vesicles. Viruses. 2018 Aug
8;10(8):415. doi: 10.3390/v10080415. PMID: 30096847; PMCID: PMC6116177.

Xiong Y, Jentzsch AV, Osterrieth JWM, Sezgin E, Sazanovich IV, Reglinski K,
Galiani S, Parker AW, Eggeling C, Anderson HL. Correction: Spironaphthoxazine
switchable dyes for biological imaging. Chem Sci. 2018 Mar 29;9(15):3892. doi:
10.1039/c8sc90071j. Erratum for: Chem Sci. 2018 Feb 20;9(11):3029-3040. doi:
10.1039/c8sc00130h. PMID: 30090292; PMCID: PMC6062417.

Lyman E, Hsieh CL, Eggeling C. From Dynamics to Membrane Organization:
Experimental Breakthroughs Occasion a "Modeling Manifesto". Biophys J. 2018 Aug
21;115(4):595-604. doi: 10.1016/j.bpj.2018.07.012. Epub 2018 Jul 21. PMID:
30075850; PMCID: PMC6103736.

Urbančič I, Garvas M, Kokot B, Majaron H, Umek P, Cassidy H, Škarabot M,
Schneider F, Galiani S, Arsov Z, Koklic T, Matallanas D, Čeh M, Muševič I,
Eggeling C, Štrancar J. Nanoparticles Can Wrap Epithelial Cell Membranes and
Relocate Them Across the Epithelial Cell Layer. Nano Lett. 2018 Aug
8;18(8):5294-5305. doi: 10.1021/acs.nanolett.8b02291. Epub 2018 Jul 26. PMID:
30039976; PMCID: PMC6089500.

van der Velde JHM, Oelerich J, Huang J, Smit JH, Jazi AA, Galiani S,
Kolmakov K, Gouridis G, Eggeling C, Herrmann A, Roelfes G, Cordes T. Author
Correction: A simple and versatile design concept for fluorophore derivatives
with intramolecular photostabilization. Nat Commun. 2018 Jul 24;9:16232. doi:
10.1038/ncomms16232. Erratum for: Nat Commun. 2016 Jan 11;7:10144. doi:
10.1038/ncomms10144. PMID: 30039809; PMCID: PMC6063355.

Mobarak E, Javanainen M, Kulig W, Honigmann A, Sezgin E, Aho N, Eggeling C,
Rog T, Vattulainen I. How to minimize dye-induced perturbations while studying
biomembrane structure and dynamics: PEG linkers as a rational alternative.
Biochim Biophys Acta Biomembr. 2018 Nov;1860(11):2436-2445. doi:
10.1016/j.bbamem.2018.07.003. Epub 2018 Jul 18. PMID: 30028957.

Schneider F, Waithe D, Lagerholm BC, Shrestha D, Sezgin E, Eggeling C,
Fritzsche M. Statistical Analysis of Scanning Fluorescence Correlation
Spectroscopy Data Differentiates Free from Hindered Diffusion. ACS Nano. 2018
Aug 28;12(8):8540-8546. doi: 10.1021/acsnano.8b04080. Epub 2018 Jul 26. PMID:
30028588; PMCID: PMC6117752.

Basu S, Needham LM, Lando D, Taylor EJR, Wohlfahrt KJ, Shah D, Boucher W,
Tan YL, Bates LE, Tkachenko O, Cramard J, Lagerholm BC, Eggeling C, Hendrich B,
Klenerman D, Lee SF, Laue ED. FRET-enhanced photostability allows improved
single-molecule tracking of proteins and protein complexes in live mammalian
cells. Nat Commun. 2018 Jun 28;9(1):2520. doi: 10.1038/s41467-018-04486-0. PMID:
29955052; PMCID: PMC6023872.

Eggeling C, Hellriegel C. Editorial. Methods. 2018 May 1;140-141:1-2. doi:
10.1016/j.ymeth.2018.04.034. PMID: 29895447.

Schneider F, Waithe D, Galiani S, Bernardino de la Serna J, Sezgin E,
Eggeling C. Nanoscale Spatiotemporal Diffusion Modes Measured by Simultaneous
Confocal and Stimulated Emission Depletion Nanoscopy Imaging. Nano Lett. 2018
Jul 11;18(7):4233-4240. doi: 10.1021/acs.nanolett.8b01190. Epub 2018 Jun 19.
PMID: 29893574; PMCID: PMC6047073.

Chojnacki J, Eggeling C. Super-resolution fluorescence microscopy studies of
human immunodeficiency virus. Retrovirology. 2018 Jun 8;15(1):41. doi:
10.1186/s12977-018-0424-3. PMID: 29884197; PMCID: PMC5994058.

Reina F, Galiani S, Shrestha D, Sezgin E, de Wit G, Cole D, Christoffer
Lagerholm B, Kukura P, Eggeling C. Complementary studies of lipid membrane
dynamics using iSCAT and super-resolved fluorescence correlation spectroscopy. J
Phys D Appl Phys. 2018 Jun 13;51(23):235401. doi: 10.1088/1361-6463/aac04f. Epub
2018 May 16. PMID: 29853718; PMCID: PMC5964363.

Xiong Y, Vargas Jentzsch A, Osterrieth JWM, Sezgin E, Sazanovich IV,
Reglinski K, Galiani S, Parker AW, Eggeling C, Anderson HL. Spironaphthoxazine
switchable dyes for biological imaging. Chem Sci. 2018 Feb 20;9(11):3029-3040.
doi: 10.1039/c8sc00130h. Erratum in: Chem Sci. 2018 Mar 29;9(15):3892. doi:
10.1039/c8sc90071j. PMID: 29732087; PMCID: PMC5916019.

Hertzog J, Dias Junior AG, Rigby RE, Donald CL, Mayer A, Sezgin E, Song C,
Jin B, Hublitz P, Eggeling C, Kohl A, Rehwinkel J. Infection with a Brazilian
isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein
blocks type I IFN induction and signaling. Eur J Immunol. 2018
Jul;48(7):1120-1136. doi: 10.1002/eji.201847483. Epub 2018 Apr 6. PMID:
29572905; PMCID: PMC6055886.

Gutowska-Owsiak D, de La Serna JB, Fritzsche M, Naeem A, Podobas EI, Leeming
M, Colin-York H, O'Shaughnessy R, Eggeling C, Ogg GS. Orchestrated control of
filaggrin-actin scaffolds underpins cornification. Cell Death Dis. 2018 Apr
1;9(4):412. doi: 10.1038/s41419-018-0407-2. PMID: 29545605; PMCID: PMC5854575.

Santos AM, Ponjavic A, Fritzsche M, Fernandes RA, de la Serna JB, Wilcock
MJ, Schneider F, Urbančič I, McColl J, Anzilotti C, Ganzinger KA, Aßmann M,
Depoil D, Cornall RJ, Dustin ML, Klenerman D, Davis SJ, Eggeling C, Lee SF.
Capturing resting T cells: the perils of PLL. Nat Immunol. 2018
Mar;19(3):203-205. doi: 10.1038/s41590-018-0048-8. Epub 2018 Feb 23. PMID:
29476188; PMCID: PMC7612954.

Waithe D, Schneider F, Chojnacki J, Clausen MP, Shrestha D, de la Serna JB,
Eggeling C. Optimized processing and analysis of conventional confocal
microscopy generated scanning FCS data. Methods. 2018 May 1;140-141:62-73. doi:
10.1016/j.ymeth.2017.09.010. Epub 2017 Sep 28. PMID: 28963070; PMCID:
PMC6026296.