Methods
- Xenograft mouse model system
- Detection of cellular senescence marker, apoptosis, stem cell character - stemness
- Production of spheroids
- Ex vivo analysis of human prostate cancer tumor samples
- Analysis of circulating tumor cells of prostate cancer patients
- RNA-seq - transcriptome analyzes
- ChIP-qPCR; ChIP-seq
Methods of DNA-protein-protein interaction:
- Chromatin Immunoprecipitation (ChIP)
- Co-immunoprecipitation
- EMSA
- Mammalian cell cultures
Hormonal regulation of gene expression:
- qRT-PCR
- DNA transfection in cell cultures
- Reporter assays