Module S3.01 - Biological microscopy
Coordinator
Prof. Dr. Christoph Biskup
Lecturers
- Biomolecular Photonics Group: Prof. Dr. Christoph Biskup, PD Dr. Christian Melle, Dr. Birgit Hoffmann
- Single-Molecule Microscopy Group: Prof. Dr. Michael Börsch
- BioImaging Group: Prof. Dr. Knut Holthoff, PD Dr. Knut Kirmse
- Institute of Molecular Cell Biology: Prof. Dr. Carsten Hoffmann
- Institute of Physiology II: Dr. Ralf Schmauder
Schedule
- Lectures: every Friday 14.15 - 15.45 (Lecture hall Humangenetik, if not stated otherwise) starting on Oct 18th.
- Exercise: every second Friday 16.00 - 17.30 (location will be announced in the lectures)
Contents
Week | Lecturer | Content | Download |
1 |
Biskup |
Introduction Techniques in fluorescence microscopy |
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2 |
Biskup Hoffmann Kache |
Practical exercises: Fluorescence microscopy |
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Please, note: On Nov 1st no lecture will take place. The lecture is postponed to Nov. 8th |
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3
08.11.2019 |
Biskup |
Fluorophores I |
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4 |
Biskup |
Fluorophores II |
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5 |
Techniques in fluorescence microscopy (Cont.) |
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6 |
Biskup |
Förster Resonance Energy Transfer (FRET) |
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16.00-17.30 |
Biskup, Hoffmann |
Practical exercises: Fluorescence intensity based FRET measurements
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Data of SE-FRET measurements acquired during the course: |
7 |
Schmauder |
Illuminating molecular processes |
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16.00-17.30 |
Biskup, Hoffmann |
Practical exercises: Fluorescence lifetime based FRET measurements |
Data of FLIM-FRET measurements acquired during the course: |
8 |
Börsch |
Single molecule measurements |
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9 |
Börsch |
Superresolution techniques |
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16.00-17.30 |
Börsch |
Practical exercises: Superresolution techniques |
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10 |
Biskup |
Evaluation of the FRET measurements Please note: Lecture is postponed to February 7th, 2020 |
|
11 |
Holthoff, Kirmse |
Fluorescent indicators |
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12 |
Holthoff, Kirmse |
Manipulation of cells and tissues with optical tools Please note: The lecture will take place in Lobeda (Forschungszentrum Lobeda, seminar room 012, ground floor. Take the hallway to your left after entering the building. The seminar room is the second room on the left side.) |
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16.00-17.30 |
Holthoff, Kirmse |
Measurements in neuronal tissue Please note: The practical part of the course will take place in the lab of Prof. Holthoff (Forschungszentrum Lobeda) |
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13 |
Hoffmann | Applications I - GPCRs Measurement of receptor activation |
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14 |
Holthoff, Kirmse |
Application II - Measurements in slices and living animals Please note: The lecture will take place in Lobeda (Forschungszentrum Lobeda, seminar room 012, ground floor) |
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16.00-17.30 |
Holthoff, Kirmse |
Discussion of the experiments of Jan 17th Please note: The practical part of the course will take place in the lab of Prof. Holthoff (Forschungszentrum Lobeda) |
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15 |
Biskup |
Applications III - Receptor channels |
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16 28.02.2020 |
Biskup |
Exam |
Requirements to complete the module:
- Evaluation of experiments performed during the course.
- Written exam at the end of the semeseter.
Exam:
February 28th, 2020, 10.00 a.m., Lecture Hall Humangenetik
Some training questions can be found here.
Recommended literature
On this page you can find links to further resources (textbooks & websites) about topics covered in the course. Publications, which you should have read before the respective lectures starts, are highlighted in red.
Please note: Linked pdfs are only accessible from computers directly connected to the computer network of the Friedrich-Schiller-University Jena.
Link | |
Optics |
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Books B.E.A. Saleh, M.C. Teich |
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Microscopy |
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Review articles |
Link
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Books James B. Pawley (Ed.) Handbook of biological confocal microscopy, 3rd ed. Springer, Singapore 2006. |
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World Wide Web |
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Fluorescence |
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Books |
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Fluorescence lifetime imaging |
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Review articles |
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Books |
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Organic fluorophores and labelling techniques | |
Review articles on labelling techniques and green fluorescent proteins Introduction into labelling techniques: B.N.G. Giepmans, S.R. Adams, M.H. Ellisman, R.Y.Tsien: |
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Genetically encoded fluorescent proteins |
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Overview about GFPs: N.C. Shaner, P.A. Steinbach, R.Y. Tsien: Discovery of GFPs: O. Shimomura: Angew. Chem. Int. Ed. Engl. 48, 5590-5602 (2009) R.Y. Tsien: See also the website of the Nobel Prize committee But see also B. Lieff Benderly: The man who wasn't there. Science-Website |
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FRET |
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Review articles C. Biskup et. al.: |