Prof. Dr. Thomas Zimmer

Wissenschaftlicher Mitarbeiter

Telefon: 03641 - 9 397655 / 9 397680
Web

Kurzbiografie

seit 1998	wissenschaftlicher Mitarbeiter am Institut für Physiologie II des Universitätsklinikums Jena
2005	Habilitation im Fach Physiologie
2006	Gastprofessur an der Universität Tokyo (Japan)
2010	Vorkliniker des Jahres
2011	Ernennung zum außerplanmäßigen Professor
2015	Vorkliniker des Jahres
2017	Janus-Cornarius-Lehrpreis der Fachschaft Medizin

Forschung

Entwicklung und Anwendung optogenetischer Tools

Spannungsgesteuerte Natriumkanäle

Ionenkanalerkrankungen

Methoden

lichtinduzierte Aktionspotentiale
Molekularbiologie (Mutagenese, RT-PCR, in vitro Transkription)
elektrophysiologische Messmethoden (Patch-Clamp, Zwei-Mikroelektroden-Voltage-Clamp)

Kooperationen

Prof. Dr. Georg Nagel, Julius-Maximilians-Universität Würzburg
PD Dr. Karl Föhr, Universitätsklinikum Ulm

Publikationen

Kukaj, T., Sattler, C., Zimmer, T., Schmauder, R., and Benndorf, K. (2023)

Kinetic fingerprinting of metabotropic glutamate receptorsCommun. Biol. 6, 104

vom Dahl, C., Müller, C.E., Berisha, X., Nagel, G., Zimmer, T. (2022)

Coupling the cardiac voltage-gated sodium channel to channelrhodopsin-2 generates novel optical switches for action potential studies.Membranes 12, 907

Gamal El-Din, T.M., Zimmer, T., Chahine, M. (2022)

Editorial: Structure Related Druggability of Voltage-Gated Sodium and Calcium Ion-Channels to Treat Diseases.

Front Pharmacol. 13:947511

Föhr, K.J., Rapp, M., Fauler, M., Zimmer, T., Jungwirth, B., Messerer, D.A.C. (2022)

Block of Voltage-Gated Sodium Channels by Aripiprazole in a State-Dependent Manner.

Int J Mol Sci. 23(21):12890

Yüksel, S., Bonus, M., Schwabe, T., Pfleger, C., Zimmer, T., Enke, U., Saß, I., Gohlke, H., Benndorf, K., and Kusch, J. (2022)

Uncoupling of Voltage- and Ligand-Induced Activation in HCN2 Channels by Glycine Inserts.

Front. Physiol. 13, 895324

Fuchs, E., Messerer, D.A.C., Karpel-Massler, G., Fauler, M., Zimmer, T., Jungwirth, B., and Föhr, K.J. (2021)

Block of Voltage-Gated Sodium Channels as a Potential Novel Anti-cancer Mechanism of TIC10

Front. Pharmacol. 12, 737637

Schirmeyer, J., Hummert, S., Eick, T., Schulz, E., Schwabe, T., Ehrlich, G., Kukaj, T., Wiegand, M., Sattler, C., Schmauder, R., Zimmer, T., Kosmalla, N., Münch, J., Bonus, M., Gohlke, H., and Benndorf, K. (2021)

Thermodynamic profile of mutual subunit control in a heteromeric receptorProc. Natl. Acad. Sci. 118, e2100469118

Föhr, K.J., Nastos, A., Fauler, M., Zimmer, T., Jungwirth, B., and Messerer, D.A.C. (2021)

Block of Voltage-Gated Sodium Channels by Atomoxetine in a State- and Use-dependent MannerFront. Pharmacol. 12, 622489

Walther, F., Feind, D., vom Dahl, C., Müller, C.E., Kukaj, T., Sattler, C., Nagel, G., Gao, S., and Zimmer, T. (2020)

Action potentials in Xenopus oocytes triggered by blue light

J. Gen. Physiol. 152, e201912489

Tarradas, A., Pinsach-Abuin, M. L., Mackintosh, C., Llorà-Batlle, O., Pérez-Serra, A., Batlle, M., Pérez-Villa, F., Zimmer, T., Garcia-Bassets, I., Brugada, R., Beltran-Alvarez, P., and Pagans, S. (2017)

Transcriptional regulation of the sodium channel gene (SCN5A) by GATA4 in human heart

J. Mol. Cell. Cardiol. 102, 74-82

Freyermuth, F., Rau, F., Kokunai, Y., Linke, T., Sellier, C., Nakamori, M., Kino, Y., Arandel, L., Jollet, A., Thibault, C., Philipps, M., Vicaire, S., Jost, B., Udd, B., Day, J. W., Duboc, D., Wahbi, K., Matsumura, T., Fujimura, H., Mochizuki, H., Deryckere, F., Kimura, T., Nukina, N., Ishiura, S., Lacroix, V., Campan-Fournier, A., Navratil, V., Chautard, E., Auboeuf, D., Horie, M., Imoto, K., Lee, K. Y., Swanson, M. S., Lopez de Munain, A., Inada, S., Itoh, H., Nakazawa, K., Ashihara, T., Wang, E., Zimmer, T., Furling, D., Takahashi, M. P., and Charlet-Berguerand, N. (2016)

Splicing misregulation of SCN5A contributes to cardiac-conduction delay and heart arrhythmia in myotonic dystrophy

Nat. Commun. 7, 11067

Nache, V., Wongsamitkul, N., Kusch, J., Zimmer, T., Schwede, F., and Benndorf, K. (2016)

Deciphering the function of the CNGB1b subunit in olfactory CNG channels

Sci. Rep. 6, 29378

Wongsamitkul, N., Nache, V., Eick, T., Hummert, S., Schulz, E., Schmauder, R., Schirmeyer, J., Zimmer, T., and Benndorf, K. (2016)

Quantifying the cooperative subunit action in a multimeric membrane receptor

Sci. Rep. 6, 20974

Zimmer, T., Haufe, V., and Blechschmidt, S. (2014)

Voltage-gated sodium channels in the mammalian heart

Global Cardiology Science and Practice 4, 58

Beltran-Alvarez, P., Espejo, A., Schmauder, R., Beltran, C., Mrowka, R., Linke, T., Batlle, M., Pérez-Villa, F., Pérez, G. J., Scornik, F. S., Benndorf, K., Pagans, S., Zimmer, T., and Brugada, R. (2013)

Protein arginine methyl transferases-3 and -5 increase cell surface expression of cardiac sodium channel

FEBS Lett. 587, 3159-65

Gütter, C., Benndorf, K., and Zimmer, T. (2013)

Characterization of N-terminally mutated cardiac Na(+) channels associated with long QT syndrome 3 and Brugada syndrome

Front Physiol. 4, 153

Nache, V., Zimmer, T., Wongsamitkul, N., Schmauder, R., Kusch, J., Reinhardt, L., Bönigk, W., Seifert, R., Biskup, C., Schwede, F., and Benndorf, K. (2012)

Differential Regulation by Cyclic Nucleotides of the CNGA4 and CNGB1b Subunits in Olfactory Cyclic Nucleotide-Gated Channels

Sci. Signal. 5, ra48

Kusch, J., Thon, S., Schulz, E., Biskup, C., Nache, V., Zimmer, T., Seifert, R., Schwede, F., and Benndorf, K. (2012)

How subunits cooperate in cAMP-induced activation of homotetrameric HCN2 channels

Nat. Chem. Biol. 8, 162-9

Walzik, S., Schroeter, A., Benndorf, K., and Zimmer, T. (2011)

Alternative splicing of the cardiac sodium channel creates multiple variants of mutant T1620K channels

PLoS One 6, e19188

Zimmer, T., and Benndorf, K. (2011)

Molecular mechanisms of voltage-gated Na+ channel dysfunction in LQT3 syndrome

In Tripathi et al. (eds.): Heart Rate and Rhythm, Springer-Verlag Berlin Heidelberg

Gui, J., Wang, T., Jones, R. P., Trump, D., Zimmer, T., and Lei, M. (2010)

Multiple loss-of-function mechanisms contribute to SCN5A-related familial sick sinus syndrome

PLoS One 5, e10985

Zimmer, T. (2010)

Effects of tetrodotoxin on the mammalian cardiovascular system

Mar. Drugs 8, 741-62

Schroeter, A., Walzik, S., Blechschmidt, S., Haufe, V., Benndorf, K., and Zimmer, T. (2010)

Structure and function of splice variants of the cardiac voltage-gated sodium channel Na(v)1.5

J. Mol. Cell. Cardiol. 49, 16-24

Gui, J., Wang, T., Trump, D., Zimmer, T., and Lei, M. (2010)

Mutation-specific effects of polymorphism H558R in SCN5A-related sick sinus syndrome

J. Cardiovasc. Electrophysiol. 21, 564-73

Kusch, J., Zimmer, T., Holschuh, J., Biskup, C., Schulz, E., Nache, V., and Benndorf. K. (2010)

Role of the S4-S5 linker in CNG channel activation

Biophys. J. 99, 2488-96

Kusch, J., Biskup, C., Thon, S., Schulz, E., Nache, V., Zimmer, T., Schwede, F., and Benndorf, K. (2010)

Interdependence of receptor activation and ligand binding in HCN2 pacemaker channels

Neuron 67, 75-85

Blechschmidt, S., Haufe, V., Benndorf, K., and Zimmer, T. (2008)

Voltage-gated Na+ channel transcript patterns in the mammalian heart are species-dependent

Prog. Biophys. Mol. Biol. 98, 309-18

Zimmer, T., and Surber, R. (2008)

SCN5A channelopathies-an update on mutations and mechanisms

Prog. Biophys. Mol. Biol. 98, 120-36

Zhang, Y., Wang, T., Ma, A., Zhou, X., Gui, J., Wan, H., Shi, R., Huang, C., Grace, A. A., Huang, C. L., Trump, D., Zhang, H., Zimmer, T., and Lei, M. (2008)

Correlations between clinical and physiological consequences of the novel mutation R878C in a highly conserved pore residue in the cardiac Na+ channel

Acta Physiol (Oxf). 194, 311-23

Surber, R., Hensellek, S., Prochnau, D., Benndorf, K., Figulla, H. R., and Zimmer, T. (2008)

Combination of cardiac conduction disease and long QT syndrome caused by a T1620K mutation in the cardiac sodium channel

Cardiovasc. Res. 77, 740-8

Hoffmann, B., Zimmer, T., Klöcker, N., Kelbauskas, L., König, K., Benndorf, K., and Biskup, C. (2008)

Prolonged irradiation of enhanced cyan fluorescent protein or Cerulean can invalidate Forster resonance energy transfer measurements

J. Biomed. Opt. 13, 031205

Nache, V., Kusch, J., Biskup, C., Schulz, E., Zimmer, T., Hagen, V., and Benndorf, K. (2008)

Thermodynamics of activation gating in olfactory-type cyclic nucleotide-gated (CNGA2) channels

Biophys. J. 95, 2750-8

Zimmer, T., and Benndorf, K. (2007)

The intracellular domain of the beta 2 subunit modulates the gating of cardiac Na v 1.5 channels

Biophys. J. 92, 3885-92

Biskup, C., Zimmer, T., Kelbauskas, L., Hoffmann, B., Klöcker, N., Becker, W., Bergmann, A., and Benndorf, K. (2007)

Multi-dimensional fluorescence lifetime and FRET measurements

Microsc. Res. Tech. 70, 442-51

Melle, C., Camacho, J. A., Surber, R., Betge, S., Eggeling, F., and Zimmer, T. (2006)

Region-specific alterations of global protein expression in the remodelled rat myocardium

Int. J. Mol. Med. 18, 1207-1215

Camacho, J. A., Hensellek, S., Rougier, J. S., Blechschmidt, S., Abriel, H., Benndorf, K., and Zimmer, T. (2006)

Modulation of Nav1.5 channel function by an alternatively spliced sequence in the DII/DIII linker region

J. Biol. Chem. 281, 9598-9506

Surber, R., Bollensdorff, C., Betge, S., Zimmer, T., and Benndorf, K. (2006)

K(ATP) channel current increases in postinfarction remodeled cardiomyocytes

Pflügers Arch. 452, 428-34

Haufe, V., Cordeiro, J. M., Zimmer, T., Wu, Y. S., Schiccitano, S., Benndorf, K., and Dumaine, R. (2005)

Contribution of neuronal sodium channels to the cardiac fast sodium current INa is greater in dog heart Purkinje fibers than in ventricles

Cardiovasc. Res. 65, 117-127

Haufe, V., Camacho, J. A., Günther, B., Dumaine, R., Bollensdorff, C., Benndorf, K., and Zimmer, T. (2005)

Expression pattern of neuronal and skeletal muscle voltage-gated Na+ channels in the developing mouse heart

J. Physiol. 564, 683-696

Nache, V., Schulz, E., Zimmer, T., Kusch, J., Biskup, C., Koopmann, R., Hagen, V., and Benndorf, K. (2005)

Activation of olfactory-type cyclic nucleotide-gated channels is highly cooperative

J. Physiol. 569, 91-102

Biskup, C., Zimmer, T., and Benndorf, K. (2004)

FRET between cardiac Na+ channel subunits measured with a confocal microscope and a streak camera

Nat. Biotechnol. 22, 220-4

Scholle, A., Zimmer, T., Koopmann, R., Engeland, B., Pongs, O., and Benndorf, K. (2004)

Effects of Kv1.2 intracellular regions on activation of Kv2.1 channels

Biophys. J. 87, 873-82

Biskup, C., Kelbauskas, L., Zimmer, T., Benndorf, K., Bergmann, A., Becker, W., Ruppersberg, J. P., Stockklausner, C., and Klöcker, N. (2004)

Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging

J. Biomed. Opt. 9, 753-9

Scholle, A., Dugarmaa, S., Zimmer, T., Leonhardt, M., Koopmann, R., Engeland, B., Pongs, O., and Benndorf, K. (2004)

Rate-limiting reactions determining different activation kinetics of Kv1.2 and Kv2.1 channels

J. Membr. Biol. 198, 103-12

Bollensdorff, C., Knopp, A., Biskup, C., Zimmer, T., and Benndorf, K. (2004)

Na(+) current through KATP channels: consequences for Na(+) and K(+) fluxes during early myocardial ischemia

Am. J. Physiol. Heart Circ. Physiol. 286, H283-95

Zimmer, T., and Benndorf, K. (2002)

The human heart and rat brain IIA Na+ channels interact with different molecular regions of the ß1 Subunit

J. Gen. Physiol. 120, 887-895

Zimmer, T., Biskup, C., Bollensdorff, C., and Benndorf, K. (2002)

The beta1 subunit but not the beta2 subunit colocalizes with the human heart sodium channel (hH1) already within the endoplasmic reticulum

J. Membrane Biol. 186, 13-21

Zimmer, T., Biskup, C., Dugarmaa, S., Vogel, F., Steinbis, M., Böhle, T., Wu, Y. S., Dumaine, R., and Benndorf, K. (2002)

Functional expression of GFP-linked human heart sodium channel (hH1) and subcellular localization of the alpha subunit in HEK293 cells and dog cardiac myocytes

J. Membrane Biol. 186, 1-12

Zimmer, T., Bollensdorff, C., Haufe, V., Birch-Hirschfeld, E., and Benndorf, K. (2002)

Mouse heart sodium channels: Primary structure and function of two different isoforms and alternatively spliced variants

Am. J. Physiol. 282, H1007-H1017

Bollensdorff, C., Zimmer, T., and Benndorf, K. (2001)

Amiloride derivatives are potent blockers of KATP channels

Naunyn Schmiedebergs Arch. Pharmacol. 364, 351-8

Möttig, H., Kusch, J., Zimmer, T., Scholle, A., and Benndorf, K. (2001)

Molecular regions controlling the activity of CNG channels

J. Gen. Physiol. 118, 183-92

Koopmann, R., Scholle, A., Ludwig, J., Leicher, T., Zimmer, T., Pongs, O., and Benndorf, K. (2001)

Role of the S2 and S3 segment in determining the activation kinetics in Kv2.1 channels

J. Membrane Biol. 182, 49-59

Zimmer, T., Ogura, A., Takewaka, T., Zimmer, R. M., Ohta, A., and Takagi, M. (2000)

Gene regulation in response to overexpression of cytochrome P450 and proliferation of the endoplasmic reticulum in Saccharomyces cerevisiae

Biosci. Biotechnol. Biochem. 64, 1930-6

Zimmer, T., Ogura, A., Ohta, A., and Takagi, M. (1999)

Misfolded membrane-bound cytochrome P450 activates KAR2 induction through two distinct mechanisms

J. Biochem. 126, 1080-9

Takewaka, T., Zimmer, T., Hirata, A., Ohta, A., and Takagi, M. (1999)

Null mutation in IRE1 gene inhibits overproduction of microsomal cytochrome P450Alk1 (CYP 52A3) and proliferation of the endoplasmic reticulum in Saccharomyces cerevisiae

J. Biochem. 125, 507-14

Sandig, G., Kärgel, E., Menzel, R., Vogel, F., Zimmer, T., and Schunck, W. H. (1999)

Regulation of endoplasmic reticulum biogenesis in response to cytochrome P450 overproduction

Drug. Metab. Rev. 31, 393-410

Scheller, U., Zimmer, T., Becher, D., Schauer, F., and Schunck, W. H. (1998)

Oxygenation cascade in conversion of n-alkanes to alpha,omega-dioic acids catalyzed by cytochrome P450 52A3

J. Biol. Chem. 273, 32528-34

Zimmer, T., Iida, T., Schunck, W. H., Yoshida, Y., Ohta, A., and Takagi, M. (1998)

Relation between evolutionary distance and enzymatic properties among the members of the CYP52A subfamily of Candida maltosa

Biochem. Biophys. Res. Commun. 251, 244-7

Zimmer, T., Scheller, U., Takagi, M., and Schunck, W. H. (1998)

Mutual conversion of fatty-acid substrate specificity by a single amino-acid exchange at position 527 in P-450Cm2 and P-450Alk3A

Eur. J. Biochem. 256, 398-403

Ohkuma, M., Zimmer, T., Iida, T., Schunck, W. H., Ohta, A., and Takagi, M. (1998)

Isozyme function of n-alkane-inducible cytochromes P450 in Candida maltosa revealed by sequential gene disruption

J. Biol. Chem. 273, 3948-53

Zimmer, T., Vogel, F., Ohta, A., Takagi, M., and Schunck, W. H. (1997)

Protein quality--a determinant of the intracellular fate of membrane-bound cytochromes P450 in yeast

DNA Cell Biol. 16, 501-14

Zimmer, T., Ohkuma, M., Ohta, A., Takagi, M., and Schunck, W. H. (1996)

The CYP52 multigene family of Candida maltosa encodes functionally diverse n-alkane-inducible cytochromes P450

Biochem. Biophys. Res. Commun. 224, 784-9

Scheller, U., Zimmer, T., Kärgel, E., and Schunck W. H. (1996)

Characterization of the n-alkane and fatty acid hydroxylating cytochrome P450 forms 52A3 and 52A4

Arch. Biochem. Biophys. 328, 245-54

Zimmer, T., Kaminski, K., Scheller, U., Vogel, F., and Schunck, W. H. (1995)

In vivo reconstitution of highly active Candida maltosa cytochrome P450 monooxygenase systems in inducible membranes of Saccharomyces cerevisiae

DNA Cell Biol. 14, 619-28

Ohkuma, M., Park, S. M., Zimmer, T., Menzel, R., Vogel, F., Schunck, W. H., Ohta, A., and Takagi, M. (1995)

Proliferation of intracellular membrane structures upon homologous overproduction of cytochrome P-450 in Candida maltosa

Biochim. Biophys. Acta 1236, 163-9

Zimmer, T., and Schunck, W. H. (1995)

A deviation from the universal genetic code in Candida maltosa and consequences for heterologous expression of cytochromes P450 52A4 and 52A5 in Saccharomyces cerevisiae

Yeast. 11, 33-41

Gutachtertätigkeit

Begutachtung von Manuskripten für wissenschaftliche Journale (Circulation, J. Biol. Chem., Prog. Biophys. Mol. Biol., J. Mol. Cell. Cardiol., J. Physiol., Pflügers Arch., J. Cell. Physiol., Neuroscience, FEBS Lett., Brit. J. Pharmacol., Int. J. Cardiol., Mol. Cell. Biochem., Case Report Med., Curr. Microbiol., Anat. Rec.)
Begutachtungen von Drittmittel- und Stipendienanträgen
Begutachtungen von Diplomarbeiten und Dissertationen

Lehrerfahrungen

Vorlesungen im Fach Physiologie für Humanmedizin- und Zahnmedizinstudenten seit 2000
Seminare im Fach Physiologie für Humanmedizin- und Zahnmedizinstudenten seit 1998
Physikumsprüfer seit 2003
Organisation der Repetitorien im Fach Physiologie seit 2009

weitere Aufgaben

Stellvertretender Institutsdirektor
S1-Projektleiter
Molekularbiologische Arbeiten des Instituts zu K⁺-Kanälen und P2X-Rezeptoren (Forschungsprojekte von Prof. Benndorf)