SP14

Principal Investigator: Otmar Huber (Institute for Biochemistry II, Jena University Hospital)

Pauline Förster (PhD): The role of Nit1 in aging-associated stress response

In this project we want to identify effectors and pathways that are regulated by Nit1 and how these effectors affect aging-associated stress responses. Using a mass spectrometry-based approach to compare cells with a stable shRNA-mediated knock-down of Nit1 with scrambled shRNA-treated control cells, we want to screen for differentially regulated proteins. After bioinformatic analysis and verification of candidates we will study the role of Nit1 in the regulation of candidates specifically in the context of our previously observed crosstalk with FOXO3a and b-catenin signaling and how Nit-dependent regulation of these factors may affect senescence and aging.