The Single-Molecule Microscopy Group studies conformational changes of single membrane enzymes (i.e. FoF1-ATP synthase) which are specifically labeled with dyes. During free diffusion through the confocal laser focus, fluorescence of the FRET donor dye (green dot, right) and FRET acceptor dye (red dot) is recorded and the actual distance calculated from the intensity ratio (green and red traces).
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