AG Klinisch-Spektroskopische Bildgebung

2024

Hopp MT, Ugurlar D, Pezeshkpoor B, Biswas A, Ramoji A, Neugebauer U, Oldenburg J, Imhof D. In-depth structure-function profiling of the complex formation between clotting factor VIII and heme. Thromb Res. 2024 May;237:184-195. doi: 10.1016/j.thromres.2024.04.006. Epub 2024 Apr 13. PMID: 38631156.

Ahmed Eltayeb S, Dressler JM, Schlatt L, Pernecker M, Neugebauer U, Karst U, Ciarimboli G. Interaction of the chemotherapeutic agent oxaliplatin and the tyrosine kinase inhibitor dasatinib with the organic cation transporter 2. Arch Toxicol. 2024 Jul;98(7):2131-2142. doi: 10.1007/s00204-024-03742-1. Epub 2024 Apr 8. PMID: 38589558; PMCID: PMC11169033.

Roth A, Tannert A, Ziller N, Eiserloh S, Göhrig B, Guliev RR, Gonzalez Vazquez MJ, Naumann M, Mosig AS, Stengel S, Heutelbeck ARR, Neugebauer U. Quantification of Polystyrene Uptake by Different Cell Lines Using Fluorescence Microscopy and Label-Free Visualization of Intracellular Polystyrene Particles by Raman Microspectroscopic Imaging. Cells. 2024 Mar 5;13(5):454. doi: 10.3390/cells13050454. PMID: 38474417; PMCID: PMC10931371.

Gandra UR, Jana B, Hammer P, Mohideen MIH, Neugebauer U, Schiller A. Lysosome targeted visible light-induced photo-CORM for simultaneous CO-release and singlet oxygen generation. Chem Commun (Camb). 2024 Feb 15;60(15):2098-2101. doi: 10.1039/d4cc00009a. PMID: 38295368.

Mandal S, Tannert A, Löffler B, Neugebauer U, Silva LB. Findaureus: An open- source application for locating Staphylococcus aureus in fluorescence-labelled infected bone tissue slices. PLoS One. 2024 Jan 31;19(1):e0296854. doi: 10.1371/journal.pone.0296854. PMID: 38295056; PMCID: PMC10830009.

2023

Steinbüchel M, Menne J, Schröter R, Neugebauer U, Schlatter E, Ciarimboli G. Regulation of Transporters for Organic Cations by High Glucose. Int J Mol Sci. 2023 Sep 13;24(18):14051. doi: 10.3390/ijms241814051. PMID: 37762353; PMCID: PMC10531077.

Osadare IE, Xiong L, Rubio I, Neugebauer U, Press AT, Ramoji A, Popp J. Raman Spectroscopy Profiling of Splenic T-Cells in Sepsis and Endotoxemia in Mice. Int J Mol Sci. 2023 Jul 27;24(15):12027. doi: 10.3390/ijms241512027. PMID: 37569403; PMCID: PMC10419286.

Aubrechtová Dragounová K, Ryabchykov O, Steinbach D, Recla V, Lindig N, González Vázquez MJ, Foller S, Bauer M, Bocklitz TW, Popp J, Rödel J, Neugebauer U. Identification of bacteria in mixed infection from urinary tract of patient's samples using Raman analysis of dried droplets. Analyst. 2023 Aug 7;148(16):3806-3816. doi: 10.1039/d3an00679d. PMID: 37463011.

Mandal S, Tannert A, Ebert C, Guliev RR, Ozegowski Y, Carvalho L, Wildemann B, Eiserloh S, Coldewey SM, Löffler B, Bastião Silva L, Hoerr V, Tuchscherr L, Neugebauer U. Insights into <i>S. aureus</i>-Induced Bone Deformation in a Mouse Model of Chronic Osteomyelitis Using Fluorescence and Raman Imaging. Int J Mol Sci. 2023 Jun 5;24(11):9762. doi: 10.3390/ijms24119762. PMID: 37298718; PMCID: PMC10254067.

Guliev RR, Vogler T, Arend N, Eiserloh S, Wiede A, Kunert T, Dinkel M, Popp J, Schie IW, Hildner K, Neugebauer U. High-throughput Raman spectroscopy allows ex vivo characterization of murine small intestinal intra-epithelial lymphocytes (IEL). Analyst. 2023 May 2;148(9):1978-1990. doi: 10.1039/d3an00074e. PMID: 37000525.

Kroll MK, Schloer S, Candan P, Korthals N, Wenzel C, Ihle H, Gilhaus K, Liedtke KR, Schöfbänker M, Surmann B, Schröter R, Neugebauer U, Mall G, Oswald S, Ludwig S, Rescher U, Vollenbröker B, Ciarimboli G. Importance of ACE2 for SARS-CoV-2 Infection of Kidney Cells. Biomolecules. 2023 Mar 3;13(3):472. doi: 10.3390/biom13030472. PMID: 36979408; PMCID: PMC10046276.

Naumann M, Arend N, Guliev RR, Kretzer C, Rubio I, Werz O, Neugebauer U. Label-Free Characterization of Macrophage Polarization Using Raman Spectroscopy. Int J Mol Sci. 2023 Jan 3;24(1):824. doi: 10.3390/ijms24010824. PMID: 36614272; PMCID: PMC9821063.

2022

Harrach S, Haag J, Steinbüchel M, Schröter R, Neugebauer U, Bertrand J, Ciarimboli G. Interaction of Masitinib with Organic Cation Transporters. Int J Mol Sci. 2022 Nov 16;23(22):14189. doi: 10.3390/ijms232214189. PMID: 36430667; PMCID: PMC9693006.

Dahms M, Eiserloh S, Rödel J, Makarewicz O, Bocklitz T, Popp J, Neugebauer U. Raman Spectroscopic Differentiation of <i>Streptococcus pneumoniae</i> From Other Streptococci Using Laboratory Strains and Clinical Isolates. Front Cell Infect Microbiol. 2022 Jul 22;12:930011. doi: 10.3389/fcimb.2022.930011. PMID: 35937698; PMCID: PMC9353136.

Unger N, Eiserloh S, Nowak F, Zuchantke S, Liebler-Tenorio E, Sobotta K, Schnee C, Berens C, Neugebauer U. Looking Inside Non-Destructively: Label-Free, Raman-Based Visualization of Intracellular <i>Coxiella burnetii</i>. Anal Chem. 2022 Mar 29;94(12):4988-4996. doi: 10.1021/acs.analchem.1c04754. Epub 2022 Mar. PMID: 35302749; PMCID: PMC8974703.

2021

Kretzer C, Shkodra B, Klemm P, Jordan PM, Schröder D, Cinar G, Vollrath A, Schubert S, Nischang I, Hoeppener S, Stumpf S, Banoglu E, Gladigau F, Bilancia R, Rossi A, Eggeling C, Neugebauer U, Schubert US, Werz O. Ethoxy acetalated dextran-based nanocarriers accomplish efficient inhibition of leukotriene formation by a novel FLAP antagonist in human leukocytes and blood. Cell Mol Life Sci. 2021 Dec 31;79(1):40. doi: 10.1007/s00018-021-04039-7. PMID: 34971430; PMCID: PMC8966466.

Pistiki A, Ramoji A, Ryabchykov O, Thomas-Rüddel D, Press AT, Makarewicz O, Giamarellos-Bourboulis EJ, Bauer M, Bocklitz T, Popp J, Neugebauer U. Biochemical Analysis of Leukocytes after In Vitro and In Vivo Activation with Bacterial and Fungal Pathogens Using Raman Spectroscopy. Int J Mol Sci. 2021 Sep 28;22(19):10481. doi: 10.3390/ijms221910481. PMID: 34638822; PMCID: PMC8508974.

Schorn S, Dicke AK, Neugebauer U, Schröter R, Friedrich M, Reuter S, Ciarimboli G. Expression and Function of Organic Cation Transporter 2 in Pancreas. Front Cell Dev Biol. 2021 May 28;9:688885. doi: 10.3389/fcell.2021.688885. PMID: 34124075; PMCID: PMC8195675.

Ramoji A, Thomas-Rüddel D, Ryabchykov O, Bauer M, Arend N, Giamarellos- Bourboulis EJ, Eugen-Olsen J, Kiehntopf M, Bocklitz T, Popp J, Bloos F, Neugebauer U. Leukocyte Activation Profile Assessed by Raman Spectroscopy Helps Diagnosing Infection and Sepsis. Crit Care Explor. 2021 May 12;3(5):e0394. doi: 10.1097/CCE.0000000000000394. PMID: 34079942; PMCID: PMC8162546.

Lüttgenau SM, Emming C, Wagner T, Harms J, Guske J, Weber K, Neugebauer U, Schröter R, Panichkina O, Pethő Z, Weber F, Schwab A, Wege AK, Nedvetsky P, Krahn MP. Pals1 prevents Rac1-dependent colorectal cancer cell metastasis by inhibiting Arf6. Mol Cancer. 2021 May 4;20(1):74. doi: 10.1186/s12943-021-01354-2. PMID: 33941200; PMCID: PMC8094600.

Huang J, Ali N, Quansah E, Guo S, Noutsias M, Meyer-Zedler T, Bocklitz T, Popp J, Neugebauer U, Ramoji A. Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application. Int J Mol Sci. 2021 Feb 22;22(4):2191. doi: 10.3390/ijms22042191. PMID: 33671841; PMCID: PMC7926873.

Grune C, Zens C, Czapka A, Scheuer K, Thamm J, Hoeppener S, Jandt KD, Werz O, Neugebauer U, Fischer D. Sustainable preparation of anti-inflammatory atorvastatin PLGA nanoparticles. Int J Pharm. 2021 Apr 15;599:120404. doi: 10.1016/j.ijpharm.2021.120404. Epub 2021 Feb 26. PMID: 33647413.

Detzel MS, Schmalohr BF, Steinbock F, Hopp MT, Ramoji A, Paul George AA, Neugebauer U, Imhof D. Revisiting the interaction of heme with hemopexin. Biol Chem. 2021 Feb 19;402(6):675-691. doi: 10.1515/hsz-2020-0347. PMID: 33581700.

2020

Lepa C, Hoppe S, Stöber A, Skryabin BV, Sievers LK, Heitplatz B, Ciarimboli G, Neugebauer U, Lindenmeyer MT, Cohen CD, Drexler HCA, Boor P, Weide T, Pavenstädt H, George B. TrkC Is Essential for Nephron Function and Trans- Activates Igf1R Signaling. J Am Soc Nephrol. 2021 Feb;32(2):357-374. doi: 10.1681/ASN.2020040424. Epub 2020 Dec 30. PMID: 33380522; PMCID: PMC8054883. 2020

Guo S, Beleites C, Neugebauer U, Abalde-Cela S, Afseth NK, Alsamad F, Anand S, Araujo-Andrade C, Aškrabić S, Avci E, Baia M, Baranska M, Baria E, Batista de Carvalho LAE, de Bettignies P, Bonifacio A, Bonnier F, Brauchle EM, Byrne HJ, Chourpa I, Cicchi R, Cuisinier F, Culha M, Dahms M, David C, Duponchel L, Duraipandian S, El-Mashtoly SF, Ellis DI, Eppe G, Falgayrac G, Gamulin O, Gardner B, Gardner P, Gerwert K, Giamarellos-Bourboulis EJ, Gizurarson S, Gnyba M, Goodacre R, Grysan P, Guntinas-Lichius O, Helgadottir H, Grošev VM, Kendall C, Kiselev R, Kölbach M, Krafft C, Krishnamoorthy S, Kubryck P, Lendl B, Loza- Alvarez P, Lyng FM, Machill S, Malherbe C, Marro M, Marques MPM, Matuszyk E, Morasso CF, Moreau M, Muhamadali H, Mussi V, Notingher I, Pacia MZ, Pavone FS, Penel G, Petersen D, Piot O, Rau JV, Richter M, Rybarczyk MK, Salehi H, Schenke- Layland K, Schlücker S, Schosserer M, Schütze K, Sergo V, Sinjab F, Smulko J, Sockalingum GD, Stiebing C, Stone N, Untereiner V, Vanna R, Wieland K, Popp J, Bocklitz T. Comparability of Raman Spectroscopic Configurations: A Large Scale Cross-Laboratory Study. Anal Chem. 2020 Dec 15;92(24):15745-15756. doi: 10.1021/acs.analchem.0c02696. Epub 2020 Nov 21. PMID: 33225709.

Tannert A, Garcia Lopez J, Petkov N, Ivanova A, Peneva K, Neugebauer U. Lysosome-targeting pH indicator based on peri-fused naphthalene monoimide with superior stability for long term live cell imaging. J Mater Chem B. 2021 Jan 7;9(1):112-124. doi: 10.1039/d0tb02208j. Epub 2020 Nov 20. PMID: 33215623.

Pilát Z, Jonáš A, Pilátová J, Klementová T, Bernatová S, Šiler M, Maňka T, Kizovský M, Růžička F, Pantůček R, Neugebauer U, Samek O, Zemánek P. Analysis of Bacteriophage-Host Interaction by Raman Tweezers. Anal Chem. 2020 Sep 15;92(18):12304-12311. doi: 10.1021/acs.analchem.0c01963. Epub 2020 Sep 1. PMID: 32815709.

Kiani M, Tannert A, Du N, Hübner U, Skorupa I, Bürger D, Zhao X, Blaschke D, Rebohle L, Cherkouk C, Neugebauer U, Schmidt OG, Schmidt H. Towards Bacteria Counting in DI Water of Several Microliters or Growing Suspension Using Impedance Biochips. Biosensors (Basel). 2020 Jul 23;10(8):82. doi: 10.3390/bios10080082. PMID: 32717845; PMCID: PMC7460460.

Arend N, Pittner A, Ramoji A, Mondol AS, Dahms M, Rüger J, Kurzai O, Schie

IW, Bauer M, Popp J, Neugebauer U. Detection and Differentiation of Bacterial and Fungal Infection of Neutrophils from Peripheral Blood Using Raman Spectroscopy. Anal Chem. 2020 Aug 4;92(15):10560-10568. doi: 10.1021/acs.analchem.0c01384. Epub 2020 Jul 23. PMID: 32613830.

Götz T, Dahms M, Kirchhoff J, Beleites C, Glaser U, Bohnert JA, Pletz MW, Popp J, Schlattmann P, Neugebauer U. Automated and rapid identification of multidrug resistant Escherichia coli against the lead drugs of acylureidopenicillins, cephalosporins, and fluoroquinolones using specific Raman marker bands. J Biophotonics. 2020 Aug;13(8):e202000149. doi: 10.1002/jbio.202000149. Epub 2020 Jun 18. PMID: 32410283.

Kleiber A, Ramoji A, Mayer G, Neugebauer U, Popp J, Henkel T. 3-Step flow focusing enables multidirectional imaging of bioparticles for imaging flow cytometry. Lab Chip. 2020 May 5;20(9):1676-1686. doi: 10.1039/d0lc00244e. PMID: 32282005.

Syllwasschy BF, Beck MS, Družeta I, Hopp MT, Ramoji A, Neugebauer U, Nozinovic S, Menche D, Willbold D, Ohlenschläger O, Kühl T, Imhof D. High- affinity binding and catalytic activity of His/Tyr-based sequences: Extending heme-regulatory motifs beyond CP. Biochim Biophys Acta Gen Subj. 2020 Jul;1864(7):129603. doi: 10.1016/j.bbagen.2020.129603. Epub 2020 Mar 29. PMID: 32234408.

Stefani N, Schroeckh V, Neugebauer U, Bohnert J, Brakhage AA. Genome Sequence of Escherichia coli KI683, Isolated from a Urosepsis Patient. Microbiol Resour Announc. 2020 Feb 27;9(9):e01297-19. doi: 10.1128/MRA.01297-19. PMID: 32107297; PMCID: PMC7046818.

Huang J, Ramoji A, Guo S, Bocklitz T, Boivin-Jahns V, Möller J, Kiehntopf M, Noutsias M, Popp J, Neugebauer U. Vibrational spectroscopy as a powerful tool for follow-up immunoadsorption therapy treatment of dilated cardiomyopathy – a case report. Analyst. 2020 Jan 21;145(2):486-496. doi: 10.1039/c9an01696a. Epub 2019 Nov 29. PMID: 31781708.

2019

Wißbrock A, Goradia NB, Kumar A, Paul George AA, Kühl T, Bellstedt P, Ramachandran R, Hoffmann P, Galler K, Popp J, Neugebauer U, Hampel K, Zimmermann B, Adam S, Wiendl M, Krönke G, Hamza I, Heinemann SH, Frey S, Hueber AJ, Ohlenschläger O, Imhof D. Structural insights into heme binding to IL-36α proinflammatory cytokine. Sci Rep. 2019 Nov 15;9(1):16893. doi: 10.1038/s41598-019-53231-0. PMID: 31729440; PMCID: PMC6858345.

Oswald S, Müller J, Neugebauer U, Schröter R, Herrmann E, Pavenstädt H, Ciarimboli G. Protein Abundance of Clinically Relevant Drug Transporters in The Human Kidneys. Int J Mol Sci. 2019 Oct 24;20(21):5303. doi: 10.3390/ijms20215303. PMID: 31653114; PMCID: PMC6862022.

Mondol AS, Töpfer N, Rüger J, Neugebauer U, Popp J, Schie IW. New perspectives for viability studies with high-content analysis Raman spectroscopy (HCA-RS). Sci Rep. 2019 Sep 2;9(1):12653. doi: 10.1038/s41598-019-48895-7. PMID: 31477762; PMCID: PMC6718400.

Englert FA, Seidel RA, Galler K, Gouveia Z, Soares MP, Neugebauer U, Clemens MG, Sponholz C, Heinemann SH, Pohnert G, Bauer M, Weis S. Labile heme impairs hepatic microcirculation and promotes hepatic injury. Arch Biochem Biophys. 2019 Sep 15;672:108075. doi: 10.1016/j.abb.2019.108075. Epub 2019 Aug 11. PMID: 31412260.

Ramoji A, Ryabchykov O, Galler K, Tannert A, Markwart R, Requardt RP, Rubio I, Bauer M, Bocklitz T, Popp J, Neugebauer U. Raman Spectroscopy Follows Time- Dependent Changes in T Lymphocytes Isolated from Spleen of Endotoxemic Mice. Immunohorizons. 2019 Feb 8;3(2):45-60. doi: 10.4049/immunohorizons.1800059. PMID: 31356153.

Töpfer N, Müller MM, Dahms M, Ramoji A, Popp J, Slevogt H, Neugebauer U. Raman spectroscopy reveals LPS-induced changes of biomolecular composition in monocytic THP-1 cells in a label-free manner. Integr Biol (Camb). 2019 Mar 1;11(3):87-98. doi: 10.1093/intbio/zyz009. PMID: 31083720.

Ramu V, Upendar Reddy G, Liu J, Hoffmann P, Sollapur R, Wyrwa R, Kupfer S, Spielmann C, Bonnet S, Neugebauer U, Schiller A. Two-Photon-Induced CO-Releasing Molecules as Molecular Logic Systems in Solution, Polymers, and Cells. Chemistry. 2019 Jun 26;25(36):8453-8458. doi: 10.1002/chem.201901396. Epub 2019 May 20. PMID: 30980425.

Tannert A, Grohs R, Popp J, Neugebauer U. Phenotypic antibiotic susceptibility testing of pathogenic bacteria using photonic readout methods: recent achievements and impact. Appl Microbiol Biotechnol. 2019 Jan;103(2):549-566. doi: 10.1007/s00253-018-9505-4. Epub 2018 Nov 15. PMID: 30443798.

2018

Pilát Z, Bernatová S, Ježek J, Kirchhoff J, Tannert A, Neugebauer U, Samek O, Zemánek P. Microfluidic Cultivation and Laser Tweezers Raman Spectroscopy of E. coli under Antibiotic Stress. Sensors (Basel). 2018 May 18;18(5):1623. doi: 10.3390/s18051623. PMID: 29783713; PMCID: PMC5982924.

Mede R, Hoffmann P, Neumann C, Görls H, Schmitt M, Popp J, Neugebauer U, Westerhausen M. Acetoxymethyl Concept for Intracellular Administration of Carbon Monoxide with Mn(CO)₃ -Based PhotoCORMs. Chemistry. 2018 Mar 2;24(13):3321-3329. doi: 10.1002/chem.201705686. Epub 2018 Feb 6. PMID: 29314301.

Schie IW, Rüger J, Mondol AS, Ramoji A, Neugebauer U, Krafft C, Popp J. High-Throughput Screening Raman Spectroscopy Platform for Label-Free Cellomics. Anal Chem. 2018 Feb 6;90(3):2023-2030. doi: 10.1021/acs.analchem.7b04127. Epub 2018 Jan 25. PMID: 29286634.

Kirchhoff J, Glaser U, Bohnert JA, Pletz MW, Popp J, Neugebauer U. Simple Ciprofloxacin Resistance Test and Determination of Minimal Inhibitory Concentration within 2 h Using Raman Spectroscopy. Anal Chem. 2018 Feb 6;90(3):1811-1818. doi: 10.1021/acs.analchem.7b03800. Epub 2018 Jan 11. PMID: 29260541.