AG Mikroskopie - Methodik
>> Forschungsschwerpunkte
- Einzelmolekül-basierte fluoreszenzspektroskopische Untersuchungen der Konformationsdynamik von FoF1-ATP Synthasen
- Konformationsdynamik einzelner G-Protein gekoppelten Rezeptoren und Transport-ATPasen (Neurotensinrezeptor1, Pgp, KdpFABC, V-ATPase) in vitro und in vivo
- polymer-basierte und exosomale native Lipid-Nanopartikel für frühe Krebszell-Diagnostik
- ps-zeitaufgelöste Einzelmolekül-FRET-Mikroskopie, FLIM, Anisotropie-Mikroskopie, Superlokalisations-Mikroskopie, STED Superresolution-Mikroskopie, Fluoreszenzkorrelationsspektroskopie
- konfokaler ABEL trap (anti-Brownian electrokinetic trap)
- NV-Zentrum-basierte Quantensensorik der Proteindynamik mittels Nanodiamanten
Publikationen
2024
Joseph J, Spantzel L, Ali M, Moonnukandathil Joseph D, Unger S, Reglinski K, Krafft C, Müller AD, Eggeling C, Heintzmann R, Börsch M, Press AT, Täuber D. Nanoscale chemical characterization of secondary protein structure of F-Actin using mid-infr ared photoinduced force microscopy (PiF-IR). Spectrochim Acta A Mol Biomol Spectrosc. 2024 Feb 5;306:123612. doi: 10.1016/j.saa.2023.123612. Epub 2023 Nov 3. PMID: 37931494.
2023
I. Pérez, T. Heitkamp, M. Börsch, Mechanism of ADP-Inhibited ATP Hydrolysis in Single Proton-Pumping FoF1-ATP Synthase Trapped in Solution 2023 Int. J. Mol. Sci. 24, 8442.
2021
Heitkamp, M. Börsch, Fast ATP-Dependent Subunit Rotation in Reconstituted FoF1‑ATP Synthase Trapped in Solution 2021 J. Phys. Chem. B 125, 7638-7650.
Heitkamp T, Börsch M. Fast ATP-Dependent Subunit Rotation in Reconstituted F<sub>o</sub>F<sub>1</sub>-ATP Synthase Trapped in Solution. J Phys Chem B. 2021 Jul 22;125(28):7638-7650. doi: 10.1021/acs.jpcb.1c02739. Epub 2021 Jul 13. PMID:34254808.
2020
Kleineberg C, Wölfer C, Abbasnia A, Pischel D, Bednarz C, Ivanov I, Heitkamp T, Börsch M, Sundmacher K, Vidaković-Koch T. Light-Driven ATP Regeneration in Diblock/Grafted Hybrid Vesicles. Chembiochem. 2020 Aug 3;21(15):2149-2160. doi: 10.1002/cbic.201900774. Epub 2020 Apr 7. PMID: 32187828; PMCID: PMC7496644.
2019
H. Sielaff, S. Yanagisawa, W. D. Frasch, W. Junge, M. Börsch, Structural asymmetry and kinetik limping of single rotary F-ATP synthases 2019 Molecules 24, 504.
2018
J.-P. Günther, M. Börsch, P. Fischer, Diffusion Measurements of Swimming Enzymes with Fluorescence Correlation Spectroscopy 2018 Acc. Chem. Res. 51, 1911-1920.
Weiss M, Frohnmayer JP, Benk LT, Haller B, Janiesch JW, Heitkamp T, Börsch M,Lira RB, Dimova R, Lipowsky R, Bodenschatz E, Baret JC, Vidakovic-Koch T,Sundmacher K, Platzman I, Spatz JP. Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics. Nat Mater. 2018 Jan;17(1):89-96. doi: 10.1038/nmat5005. Epub 2017 Oct 16. PMID: 29035355.
Heitkamp T, Grisshammer R, Börsch M. Towards monitoring conformational changes of the GPCR neurotensin receptor 1 by single-molecule FRET. Proc SPIE Int Soc Opt Eng. 2018 Jan-Feb;10498:104980T. doi: 10.1117/12.2286787. Epub 2018 Feb 23. PMID: 30013286; PMCID: PMC6044442.
2017
Geiss AF, Khandelwal R, Baurecht D, Bliem C, Reiner-Rozman C, Boersch M, Ullmann GM, Loew LM, Naumann RL. pH and Potential Transients of the bc<sub>1</sub> Complex Co-Reconstituted in Proteo-Lipobeads with the Reaction Center from Rb. sphaeroides. J Phys Chem B. 2017 Jan 12;121(1):143-152. doi: 10.1021/acs.jpcb.6b11116. Epub 2017 Jan 4. PMID: 27992230; PMCID: PMC5699462.
Geiss AF, Bliem C, Frank P, Reiner-Rozman C, Kewney J, Boersch M, Naumann RLC. Proteo-lipobeads to encapsulate cytochrome c oxidase from Paracoccus denitrificans. J Colloid Interface Sci. 2017 Aug 15;500:119-125. doi: 10.1016/j.jcis.2017.04.009. Epub 2017 Apr 5. PMID: 28407595.