AG Mikroskopie - Methodik
>> Forschungsschwerpunkte
- Einzelmolekül-basierte fluoreszenzspektroskopische Untersuchungen der Konformationsdynamik von FoF1-ATP Synthasen
- Konformationsdynamik einzelner G-Protein gekoppelten Rezeptoren und Transport-ATPasen (Neurotensinrezeptor1, Pgp, KdpFABC, V-ATPase) in vitro und in vivo
- polymer-basierte und exosomale native Lipid-Nanopartikel für frühe Krebszell-Diagnostik
- ps-zeitaufgelöste Einzelmolekül-FRET-Mikroskopie, FLIM, Anisotropie-Mikroskopie, Superlokalisations-Mikroskopie, STED Superresolution-Mikroskopie, Fluoreszenzkorrelationsspektroskopie
- konfokaler ABEL trap (anti-Brownian electrokinetic trap)
- NV-Zentrum-basierte Quantensensorik der Proteindynamik mittels Nanodiamanten
Publikationen
2023
I. Pérez, T. Heitkamp, M. Börsch, Mechanism of ADP-Inhibited ATP Hydrolysis in Single Proton-Pumping FoF1-ATP Synthase Trapped in Solution 2023 Int. J. Mol. Sci. 24, 8442.
2021
Heitkamp, M. Börsch, Fast ATP-Dependent Subunit Rotation in Reconstituted FoF1‑ATP Synthase Trapped in Solution 2021 J. Phys. Chem. B 125, 7638-7650.
Heitkamp T, Börsch M. Fast ATP-Dependent Subunit Rotation in Reconstituted F<sub>o</sub>F<sub>1</sub>-ATP Synthase Trapped in Solution. J Phys Chem B. 2021 Jul 22;125(28):7638-7650. doi: 10.1021/acs.jpcb.1c02739. Epub 2021 Jul 13. PMID:34254808.
2020
Kleineberg C, Wölfer C, Abbasnia A, Pischel D, Bednarz C, Ivanov I, Heitkamp T, Börsch M, Sundmacher K, Vidaković-Koch T. Light-Driven ATP Regeneration in Diblock/Grafted Hybrid Vesicles. Chembiochem. 2020 Aug 3;21(15):2149-2160. doi: 10.1002/cbic.201900774. Epub 2020 Apr 7. PMID: 32187828; PMCID: PMC7496644.
2019
H. Sielaff, S. Yanagisawa, W. D. Frasch, W. Junge, M. Börsch, Structural asymmetry and kinetik limping of single rotary F-ATP synthases 2019 Molecules 24, 504.
2018
J.-P. Günther, M. Börsch, P. Fischer, Diffusion Measurements of Swimming Enzymes with Fluorescence Correlation Spectroscopy 2018 Acc. Chem. Res. 51, 1911-1920.
Weiss M, Frohnmayer JP, Benk LT, Haller B, Janiesch JW, Heitkamp T, Börsch M,Lira RB, Dimova R, Lipowsky R, Bodenschatz E, Baret JC, Vidakovic-Koch T,Sundmacher K, Platzman I, Spatz JP. Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics. Nat Mater. 2018 Jan;17(1):89-96. doi: 10.1038/nmat5005. Epub 2017 Oct 16. PMID: 29035355.
Heitkamp T, Grisshammer R, Börsch M. Towards monitoring conformational changes of the GPCR neurotensin receptor 1 by single-molecule FRET. Proc SPIE Int Soc Opt Eng. 2018 Jan-Feb;10498:104980T. doi: 10.1117/12.2286787. Epub 2018 Feb 23. PMID: 30013286; PMCID: PMC6044442.
2017
Geiss AF, Khandelwal R, Baurecht D, Bliem C, Reiner-Rozman C, Boersch M, Ullmann GM, Loew LM, Naumann RL. pH and Potential Transients of the bc<sub>1</sub> Complex Co-Reconstituted in Proteo-Lipobeads with the Reaction Center from Rb. sphaeroides. J Phys Chem B. 2017 Jan 12;121(1):143-152. doi: 10.1021/acs.jpcb.6b11116. Epub 2017 Jan 4. PMID: 27992230; PMCID: PMC5699462.
Geiss AF, Bliem C, Frank P, Reiner-Rozman C, Kewney J, Boersch M, Naumann RLC. Proteo-lipobeads to encapsulate cytochrome c oxidase from Paracoccus denitrificans. J Colloid Interface Sci. 2017 Aug 15;500:119-125. doi: 10.1016/j.jcis.2017.04.009. Epub 2017 Apr 5. PMID: 28407595.